AS-8351

2-Hydroxy-1-naphthylaldehyde isonicotinoylhydrazone; NSC-51355

AS-8351 has been used in a cocktail of nine small molecules used to efficiently induce cardiac reprogramming of human fibroblasts1. It is believed that AS-8351exerts its effects by modulating the activity of the JmjC domain-containing histone demethylases (JmjC-KDM). Thus each of the 22 genes in the JmjC-KDM family was abrogated using small hairpin RNAs which showed that only knocking down KDM5B (or using another KDM5B inhibitor such as PBIT) could phenocopy AS-8351 in generating cardiomyocytes.

Physical Properties: Yellow powder

MW=291.3 C₁₇H₁₃N₃O₂

Analysis: >98% (TLC); NMR (Conforms)

Solubilization: May be dissolved in DMSO (45 mg/ml); or ethanol (10 mg/ml, warm); or DMF (30 mg/ml)

Storage: Store desiccated as supplied at -20°C for up to 2 years. Store solutions at -20°C for up to 1 month.

Amount: 5 mg